P.O. Box 5982, Morogoro Road, near Dart Kisutu — City Centre, Dar es Salaam
NG Biotech
Model: NGB-CTM-S23-016
Multiplex lateral flow immunoassay that detects the five major CTX-M extended-spectrum beta-lactamase (ESBL) groups — 1, 2, 8, 9 and 25 — from an Enterobacterales colony or a positive blood culture in under 15 minutes.
ESBL resistance detection
NG-TEST® CTX-M Multi is a qualitative multiplex lateral flow immunoassay for the detection of the five major CTX-M extended-spectrum β-lactamase groups — 1, 2, 8, 9 and 25 — straight from a bacterial colony or a positive blood culture. No instrument, no molecular platform, no batching.
Group 1
CTX-M-1, -3, -15 and related variants — the most widespread CTX-M group worldwide
Group 2
CTX-M-2 and related variants, common in Latin America and spreading globally
Group 8
CTX-M-8 lineage, increasingly reported beyond its original regions
Group 9
CTX-M-9, -14, -27 and related variants — dominant in Europe and Asia
Group 25
CTX-M-94, -100 and related variants within the group 25 lineage
Why it matters
WHO Bacterial Priority Pathogens List, 2024
Third-generation cephalosporin-resistant Enterobacterales — a resistance pattern driven mainly by ESBL production — are placed in the critical priority group, alongside carbapenem-resistant Enterobacterales and Acinetobacter baumannii.
Read the sourceGRAM analysis, published in The Lancet (2024)
The Global Research on Antimicrobial Resistance study estimated 1.14 million deaths directly attributable to bacterial antimicrobial resistance in 2021, with 4.71 million deaths associated with it, and projected 1.91 million attributable deaths a year by 2050 on current trends.
Read the sourceWHO GLASS — Global AMR and Use Surveillance System
WHO reports high and rising levels of resistance to third-generation cephalosporins in E. coli and Klebsiella pneumoniae from bloodstream infections, and notes that national surveillance depends on laboratories able to detect resistance reliably.
Read the sourceWHO Global Action Plan on AMR
Optimising the use of antimicrobial medicines — one of the plan's five strategic objectives — requires knowing whether a patient's isolate carries an ESBL before broad-spectrum agents such as carbapenems are escalated unnecessarily.
Read the sourceWHO list of critically important antimicrobials
WHO classifies third- and fourth-generation cephalosporins as highest-priority critically important antimicrobials for human medicine, making ESBL detection central to protecting their usefulness.
Read the sourceWHO / Africa CDC on AMR surveillance capacity
Both organisations identify limited laboratory and diagnostic capacity as a central constraint on AMR surveillance and treatment decisions across Africa, and prioritise strengthening it at national and referral laboratory level.
Read the sourceSummaries of publicly available material from WHO, Africa CDC and peer-reviewed research, provided as background on antimicrobial resistance. They are not claims about this product and do not constitute an endorsement of any device by those organisations.
Workflow
Use a pure colony of Enterobacterales — such as E. coli or Klebsiella pneumoniae — from selective medium, or go directly from a positive blood culture.
Add the supplied buffer to the microtube, transfer the colonies with the loop or pipette provided and mix.
Transfer the suspension into the sample well of the single-use cassette. No calibration or reagent preparation.
Read the control line plus the five group test lines visually. Each positive line identifies a CTX-M ESBL group directly.
Clinical value
Group-level ESBL identification within the same shift, instead of waiting on phenotypic confirmation or molecular batch runs.
Run directly from positive blood cultures — paired with NG-TEST Blood Culture PREP — to support treatment decisions one to two days faster than routine methods.
Confirming a CTX-M ESBL early supports targeted therapy and helps avoid unnecessary escalation to carbapenems.
Prompt flagging of ESBL-producing Enterobacterales supports isolation, cohorting and contact precautions.
Fits any laboratory setting with minimal hands-on time — no analyser, service contract or dedicated molecular room.
Group-level ESBL results feed hospital dashboards and GLASS-aligned national AMR reporting.
Interpretation
Groups 1, 2, 8, 9 and 25
CTX-M enzymes have become the most prevalent ESBL type worldwide, displacing older TEM and SHV variants. Detecting the group confirms an ESBL mechanism and informs therapy and infection-control decisions immediately.
Pairs with NG-TEST CARBA-5
Third-generation cephalosporin resistance does not say which enzyme is responsible. Run alongside NG-TEST CARBA-5 to distinguish ESBL producers from carbapenemase producers — a distinction that changes the treatment pathway.
Coverage
At a glance
| Product | NG-TEST® CTX-M Multi (NG Biotech, France) |
|---|---|
| Reference | NGB-CTM-S23-016 — kit of 20 tests |
| Technology | Multiplex immunochromatographic lateral flow assay, visual read |
| Targets | CTX-M ESBL groups 1, 2, 8, 9 and 25 |
| Sample types | Pure bacterial colonies of Enterobacterales (including E. coli and Klebsiella pneumoniae); positive blood cultures |
| Time to result | Under 15 minutes; minimal hands-on time |
| Performance | Sensitivity 100%, specificity 100% (manufacturer data) |
| Kit contents | 20 cassettes, buffer, 20 microtubes, 20 pipettes |
| Equipment needed | None — no instrumentation, calibration or reagent preparation |
| Intended use | Rapid in vitro diagnostic, professional laboratory use only |
Specifications follow the manufacturer documentation. See the product labelling for the full instructions for use, warnings and limitations for your region.
Good to know
Ordering
Each kit contains 20 single-use cassettes, buffer, 20 microtubes and 20 pipettes. Nothing else is needed to run the test.
Where it fits
Confirm CTX-M ESBL groups without a molecular platform.
Rapid ESBL answers for severe infections where empirical therapy must be narrowed fast.
Early identification of ESBL carriers to trigger isolation and contact precautions.
Consistent group-level ESBL data for GLASS-aligned reporting.
One resistance pathway
These NG Biotech assays are designed to work together: prepare the sample straight from a positive blood culture, then detect ESBL, carbapenemase and colistin-resistance mechanisms in minutes — no molecular platform required.
CarbapenemasesDetects and differentiates the five major carbapenemase families — KPC, OXA-48-like, VIM, IMP and NDM — in under 15 minutes.
View product
Colistin resistanceDetects the MCR-1 enzyme behind plasmid-mediated colistin resistance in Gram-negative bacteria in under 15 minutes.
View product
Sample preparationTurns a positive blood culture into a test-ready sample in about 20 minutes, skipping the overnight subculture step.
View productSupplied and supported in Tanzania
Our team advises on kit volumes, delivery and laboratory implementation for the NG-TEST CTX-M Multi Rapid ESBL Detection Test.
Specifications
| Targets | CTX-M extended-spectrum beta-lactamase (ESBL) groups 1, 2, 8, 9 and 25 |
|---|---|
| Ordering | Reference NGB-CTM-S23-016 — kit of 20 tests |
| Reference | NGB-CTM-S23-016 |
| Technology | Multiplex immunochromatographic lateral flow assay (visual read, no instrument) |
| Limitations | Detects the five listed CTX-M groups only; does not replace antimicrobial susceptibility testing |
| Sample type | Pure bacterial colonies of Enterobacterales (including Escherichia coli and Klebsiella pneumoniae); also positive blood cultures |
| Sensitivity | 100% |
| Specificity | 100% |
| Intended use | Rapid in vitro diagnostic assay for professional laboratory use only |
| Kit contents | 20 cassettes, buffer, 20 microtubes, 20 pipettes |
| Manufacturer | NG Biotech, France |
| Hands-on time | Minimal hands-on time with a simple preparation protocol |
| Time to result | Less than 15 minutes |
| Instrumentation | None required — no analyser, calibration or reagent preparation |
| Blood culture use | Can be run directly from positive blood cultures, in the NG-TEST Blood Culture PREP pathway |
| Detected variants | Group 1: CTX-M-1, -3, -10, -15, -32, -37, -55, -57, -71, -82, -101, -182; Group 2: CTX-M-2; Group 8: CTX-M-8; Group 9: CTX-M-9, -13, -14, -17, -18, -19, -24, -27, -38, -65, -93; Group 25: CTX-M-94, -100 |
Why Anudha
Sourced directly through our manufacturer partnerships and sole distributorships.
Our engineers advise on selection, installation and after-sales servicing.
Delivered to hospitals, clinics and laboratories across Tanzania and East Africa.
Trusted by healthcare facilities since 1992 for equipment and consumables.
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Sample preparation reagent kit that turns a positive blood culture into a test-ready bacterial sample in about 20 minutes — skipping subculture — for direct rapid detection of ESBLs and carbapenemases with NG-TEST CTX-M Multi and NG-TEST CARBA-5.
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