P.O. Box 5982, Morogoro Road, near Dart Kisutu — City Centre, Dar es Salaam
NG Biotech
Model: NGP-BCP-S73-001
Sample preparation reagent kit that turns a positive blood culture into a test-ready bacterial sample in about 20 minutes — skipping subculture — for direct rapid detection of ESBLs and carbapenemases with NG-TEST CTX-M Multi and NG-TEST CARBA-5.
Blood culture sample preparation
NG-TEST® Blood Culture PREP is a ready-to-use reagent kit that prepares bacterial samples directly from positive blood cultures — skipping the overnight subculture step — so NG-TEST rapid assays can detect carbapenemases and ESBLs one to two days earlier than routine methods.
Skip subculture
Works directly from a positive blood culture — no overnight plating step
Under 5 min prep
Lysis and washing buffers prepare the bacterial sample in minutes
~20 min total
From positive blood culture to a resistance result with a NG-TEST rapid assay
1–2 days earlier
Answers reach the clinical team one to two days sooner than routine methods
Why it matters
WHO Global Report on the Epidemiology and Burden of Sepsis, 2020
WHO estimates 49 million sepsis cases and 11 million sepsis-related deaths annually — around one in five deaths worldwide — and stresses that early, appropriate antimicrobial therapy is critical to survival.
Read the sourceWHO Bacterial Priority Pathogens List, 2024
The critical priority group — carbapenem-resistant and third-generation cephalosporin-resistant Enterobacterales and Acinetobacter baumannii — is dominated by pathogens behind severe bloodstream infections.
Read the sourceGRAM analysis, published in The Lancet (2024)
The Global Research on Antimicrobial Resistance study estimated 1.14 million deaths directly attributable to bacterial antimicrobial resistance in 2021, with bloodstream infections among the deadliest syndromes.
Read the sourceWHO GLASS — Global AMR and Use Surveillance System
WHO's surveillance of bloodstream infections reports high resistance levels in common pathogens, and states that national surveillance depends on laboratories able to detect resistance rapidly and reliably.
Read the sourceWHO Global Action Plan on AMR
Optimising antimicrobial use — a strategic objective of the plan — depends on diagnostic results arriving early enough to shape the first treatment decision, not just to confirm it days later.
Read the sourceWHO / Africa CDC on AMR surveillance capacity
Both organisations identify limited laboratory and diagnostic capacity as a central constraint on AMR surveillance and treatment decisions across Africa, and prioritise strengthening it at national and referral laboratory level.
Read the sourceSummaries of publicly available material from WHO, Africa CDC and peer-reviewed research, provided as background on antimicrobial resistance. They are not claims about this product and do not constitute an endorsement of any device by those organisations.
Workflow
Start from a flagged positive blood culture with Enterobacterales or Pseudomonas aeruginosa from a patient with suspected bloodstream infection.
Add the ready-to-use lysis buffer to release the bacteria from the blood culture matrix — no reagent preparation needed.
Use the washing buffer to clean up the bacterial sample in the supplied microtube — preparation takes under five minutes.
Load the prepared sample onto NG-TEST CARBA-5 or NG-TEST CTX-M Multi and read the resistance result — about 20 minutes in total.
Clinical value
Removing the overnight subculture step brings the resistance result forward by one to two days compared with routine workflows.
No plating, no overnight incubation — the positive blood culture bottle feeds the rapid test directly.
In bloodstream infections every hour of appropriate therapy matters — earlier resistance data supports earlier targeted treatment.
Ready-to-use buffers and microtubes run on the benchtop — no additional instrumentation or dedicated room.
A short, simple preparation protocol fits into existing blood culture workflows without adding staff burden.
Manufacturer validation with NG-TEST CARBA-5 and NG-TEST CTX-M Multi reports 100% sensitivity and specificity with both assays.
Compatibility
Carbapenemases — KPC, OXA-48-like, VIM, IMP, NDM
Prepare the blood culture sample with Blood Culture PREP, then identify and differentiate the five major carbapenemase families in under 15 minutes.
View the paired testESBLs — CTX-M groups 1, 2, 8, 9 and 25
The same prepared sample feeds the CTX-M Multi cassette for group-level ESBL identification, completing the resistance picture from one blood culture.
View the paired testGood to know
At a glance
| Product | NG-TEST® Blood Culture PREP (NG Biotech, France) |
|---|---|
| Reference | NGP-BCP-S73-001 — kit for 20 preparations |
| Product type | Sample preparation reagent kit (lysis and washing buffers, ready to use) |
| Compatible tests | NG-TEST CARBA-5 and NG-TEST CTX-M Multi — 100% sensitivity and specificity with both (manufacturer data) |
| Sample type | Positive blood cultures with Enterobacterales or Pseudomonas aeruginosa from patients with suspected bloodstream infection |
| Preparation time | Under 5 minutes; about 20 minutes to a resistance result with the rapid test |
| Kit contents | 20 microtubes, 1 lysis buffer solution, 1 washing buffer solution |
| Equipment needed | None — no additional space or instrumentation |
| Intended use | Professional laboratory use only |
Specifications follow the manufacturer documentation. See the product labelling for the full instructions for use, warnings and limitations for your region.
Ordering
Each kit contains 20 microtubes, one lysis buffer solution and one washing buffer solution. Nothing else is needed to prepare the sample.
The workflow gain
Routine blood culture workups plate the positive bottle and incubate overnight before any resistance testing can begin. Blood Culture PREP removes that step: the prepared sample goes straight onto NG-TEST CARBA-5 or NG-TEST CTX-M Multi, so the laboratory reports a resistance mechanism one to two days earlier — while the patient is still on empirical therapy.
Where it fits
Shorten the positive blood culture workflow without new equipment.
Earlier resistance data for the most critical bloodstream infections.
Faster flagging of resistant organisms from blood cultures to trigger precautions.
Supports antimicrobial stewardship where every hour of appropriate therapy counts.
One resistance pathway
These NG Biotech assays are designed to work together: prepare the sample straight from a positive blood culture, then detect ESBL, carbapenemase and colistin-resistance mechanisms in minutes — no molecular platform required.
CarbapenemasesDetects and differentiates the five major carbapenemase families — KPC, OXA-48-like, VIM, IMP and NDM — in under 15 minutes.
View product
ESBL enzymesIdentifies the five major CTX-M extended-spectrum β-lactamase groups from a colony or positive blood culture in under 15 minutes.
View product
Colistin resistanceDetects the MCR-1 enzyme behind plasmid-mediated colistin resistance in Gram-negative bacteria in under 15 minutes.
View productSupplied and supported in Tanzania
Our team advises on kit volumes, delivery and laboratory implementation for the NG-TEST Blood Culture PREP Sample Preparation Kit.
Specifications
| Purpose | Prepares a bacterial sample from a positive blood culture for rapid lateral flow resistance testing |
|---|---|
| Ordering | Reference NGP-BCP-S73-001 — kit for 20 preparations |
| Reference | NGP-BCP-S73-001 |
| Limitations | A preparation reagent, not a standalone diagnostic; always used together with a compatible NG-TEST rapid assay |
| Sample type | Positive blood cultures with Enterobacterales or Pseudomonas aeruginosa from patients with suspected bloodstream infection |
| Intended use | For professional laboratory use only |
| Kit contents | 20 microtubes, 1 lysis buffer solution, 1 washing buffer solution |
| Manufacturer | NG Biotech, France |
| Product type | Sample preparation reagent kit (lysis and washing buffers, ready to use) |
| Time to result | Sample preparation in under 5 minutes; resistance result in about 20 minutes with the rapid test |
| Instrumentation | None required — no additional space or equipment |
| Compatible tests | Validated with NG-TEST CARBA-5 and NG-TEST CTX-M Multi (100% sensitivity and specificity with both, manufacturer data) |
| Workflow benefit | Skips the overnight subculture step, supporting results 1 to 2 days earlier than routine methods |
Why Anudha
Sourced directly through our manufacturer partnerships and sole distributorships.
Our engineers advise on selection, installation and after-sales servicing.
Delivered to hospitals, clinics and laboratories across Tanzania and East Africa.
Trusted by healthcare facilities since 1992 for equipment and consumables.
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