P.O. Box 5982, Morogoro Road, near Dart Kisutu — City Centre, Dar es Salaam
NG Biotech
Model: NGB-CAR-S23-021
Lateral flow immunoassay that detects and differentiates the five most prevalent carbapenemase families — KPC, OXA-48-like, VIM, IMP and NDM — from a bacterial colony in less than 15 minutes.
Brochures and datasheets for this product are available on request — use the quote form or WhatsApp above and our team will send them across.
Carbapenemase resistance detection
NG-TEST® CARBA-5® is a qualitative lateral flow immunoassay for the detection and differentiation of the five most prevalent carbapenemase families — NDM, IMP, VIM, OXA-48-like and KPC — and their most clinically relevant variants, straight from a bacterial colony or a positive blood culture. No instrument, no molecular platform, no batching.
KPC
Klebsiella pneumoniae carbapenemase
OXA-48-like
Oxacillinase-48 family
VIM
Verona integron-encoded MBL
IMP
Imipenemase MBL
NDM
New Delhi metallo-β-lactamase
Why it matters
WHO Bacterial Priority Pathogens List, 2024
Carbapenem-resistant Enterobacterales and carbapenem-resistant Acinetobacter baumannii are placed in the critical priority group — the pathogens WHO considers the most urgent targets for new treatments and for diagnostic and surveillance investment.
Read the sourceGRAM analysis, published in The Lancet (2024)
The Global Research on Antimicrobial Resistance study estimated 1.14 million deaths directly attributable to bacterial antimicrobial resistance in 2021, with 4.71 million deaths associated with it, and projected 1.91 million attributable deaths a year by 2050 on current trends.
Read the sourceWHO GLASS — Global AMR and Use Surveillance System
WHO reports high and rising resistance among common bloodstream and urinary pathogens, and states that national surveillance depends on laboratories able to detect and characterise resistance reliably.
Read the sourceWHO Global Action Plan on AMR
Two of the plan's five strategic objectives — strengthening the evidence base through surveillance and research, and optimising the use of antimicrobial medicines — both depend on knowing which resistance mechanism a patient is carrying.
Read the sourceWHO guidelines on carbapenem-resistant organisms in health facilities
WHO recommends early identification of patients colonised or infected with carbapenem-resistant organisms, together with contact precautions, cohorting and hand-hygiene compliance, as core measures to prevent transmission.
Read the sourceWHO / Africa CDC on AMR surveillance capacity
Both organisations have repeatedly identified limited laboratory and diagnostic capacity as a central constraint on AMR surveillance and treatment decisions across Africa, and prioritise strengthening that capacity at national and referral laboratory level.
Read the sourceSummaries of publicly available material from WHO, Africa CDC and peer-reviewed research, provided as background on antimicrobial resistance. They are not claims about this product and do not constitute an endorsement of any device by those organisations.
Workflow
Use a carbapenem non-susceptible pure colony of Enterobacterales or Pseudomonas aeruginosa from selective medium — or go directly from a positive blood culture.
Add extraction buffer to the microtube, transfer the colonies with the supplied loop or pipette and mix.
Transfer 100 µl of the suspension into the sample well of the single-use cassette. No calibration or reagent preparation.
Read the control line plus the five named test lines visually. Each line identifies a carbapenemase family, so the result is directly actionable.
Clinical value
A result within the same shift, instead of waiting on phenotypic confirmation or a molecular batch run.
Run directly from positive blood cultures to support treatment decisions one to two days faster than routine methods allow.
Serine carbapenemases and metallo-β-lactamases respond to different agents — the family name changes the treatment conversation.
Prompt isolation, cohorting and contact precautions for confirmed carbapenemase producers.
Fits any laboratory setting with about two minutes of hands-on time — no analyser, service contract or dedicated molecular room.
Family-level results feed hospital dashboards and GLASS-aligned national AMR reporting.
Interpretation
KPC, OXA-48-like
Hydrolyse carbapenems through a serine active site. Newer β-lactam / β-lactamase-inhibitor combinations are generally active against this group, so identifying it opens treatment options that metallo-enzymes rule out.
NDM, VIM, IMP
Zinc-dependent enzymes that are not inhibited by classical or newer serine β-lactamase inhibitors. Detecting them redirects therapy early and flags a high infection-control priority.
Coverage
At a glance
| Product | NG-TEST® CARBA-5® (NG Biotech, France) |
|---|---|
| Reference | NGB-CAR-S23-021 — kit of 20 tests |
| Technology | Multiplex immunochromatographic lateral flow assay, visual read |
| Targets | KPC, OXA-48-like, VIM, IMP, NDM |
| Test lines | Control line plus K, O, V, I and N test lines |
| Sample types | Carbapenem non-susceptible pure colonies of Enterobacterales and Pseudomonas aeruginosa; positive blood cultures |
| Validated media | TSA, Mueller Hinton, ChromID® CARBA SMART, Drigalski (DRIG), CHROMagar™ mSuperCARBA and others |
| Time to result | Under 15 minutes; around 2 minutes hands-on |
| Performance | Sensitivity 100%, specificity 100% (manufacturer data) |
| Kit contents | 20 cassettes, extraction buffer, 20 microtubes, 20 pipettes |
| Equipment needed | None — no instrumentation, calibration or reagent preparation |
| Intended use | Rapid in vitro diagnostic, professional laboratory use only |
Specifications follow the manufacturer documentation. See the product labelling for the full instructions for use, warnings and limitations for your region.
Good to know
Ordering
Each kit contains 20 single-use cassettes, extraction buffer, 20 microtubes and 20 pipettes. Nothing else is needed to run the test.
Where it fits
Confirm and differentiate carbapenemase families without a molecular platform.
Rapid answers for severe infections where empirical therapy must be narrowed fast.
Early identification of carriers to trigger isolation and contact precautions.
Consistent family-level resistance data for GLASS-aligned reporting.
One resistance pathway
These NG Biotech assays are designed to work together: prepare the sample straight from a positive blood culture, then detect ESBL, carbapenemase and colistin-resistance mechanisms in minutes — no molecular platform required.
ESBL enzymesIdentifies the five major CTX-M extended-spectrum β-lactamase groups from a colony or positive blood culture in under 15 minutes.
View product
Colistin resistanceDetects the MCR-1 enzyme behind plasmid-mediated colistin resistance in Gram-negative bacteria in under 15 minutes.
View product
Sample preparationTurns a positive blood culture into a test-ready sample in about 20 minutes, skipping the overnight subculture step.
View productSupplied and supported in Tanzania
Our team advises on kit volumes, delivery and laboratory implementation for the NG-TEST CARBA-5 Rapid Carbapenemase Detection Test.
Specifications
| Format | Single-use cassette, 20 tests per kit |
|---|---|
| Storage | Store the kit as stated on the labelling; do not use beyond the printed expiry date |
| Targets | KPC, OXA-48-like, VIM, IMP, NDM carbapenemase families |
| Ordering | Reference NGB-CAR-S23-021 — kit of 20 tests |
| Reference | NGB-CAR-S23-021 |
| Technology | Multiplex immunochromatographic lateral flow assay (visual read, no instrument) |
| Test lines | Control line plus five named test lines: K (KPC), O (OXA-48-like), V (VIM), I (IMP), N (NDM) |
| Limitations | Detects the five listed carbapenemase families only; does not replace antimicrobial susceptibility testing |
| Read window | Visual read at 15 minutes |
| Sample type | Carbapenem non-susceptible pure bacterial colonies of Enterobacterales and Pseudomonas aeruginosa; also positive blood cultures |
| Sensitivity | 100% |
| Specificity | 100% |
| Intended use | Rapid in vitro diagnostic assay for professional laboratory use only |
| Kit contents | 20 cassettes, extraction buffer, 20 microtubes, 20 pipettes |
| Manufacturer | NG Biotech, France |
| Hands-on time | Approximately 2 minutes of preparation per sample |
| Time to result | Less than 15 minutes |
| Instrumentation | None required — no analyser, calibration or reagent preparation |
| Blood culture use | Can be run from positive blood cultures, in the NG-TEST Blood Culture PREP pathway |
| Culture media validated | TSA, Mueller Hinton, ChromID CARBA SMART, Drigalski (DRIG), CHROMagar mSuperCARBA and others |
Why Anudha
Sourced directly through our manufacturer partnerships and sole distributorships.
Our engineers advise on selection, installation and after-sales servicing.
Delivered to hospitals, clinics and laboratories across Tanzania and East Africa.
Trusted by healthcare facilities since 1992 for equipment and consumables.
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